| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099181 |
| Chromosome: | chromosome 1 |
| Location: | 2371346 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013000 | (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTAGGGGTGCCGGGGTACGACAGCGG |
| Internal bar code: | CTATAAAGCGACGGTGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 87.8477 |
| LEAP-Seq n confirming: | 26494 |
| LEAP-Seq n nonconfirming: | 3665 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCG |
| Suggested primer 2: | CATGCGATATGAATTGCCAG |