| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099187 |
| Chromosome: | chromosome 12 |
| Location: | 5315994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529150 | PPP40,PP2C1,FAP314 | Flagellar Associated Protein 314; (1 of 1) PTHR13832:SF352 - PROTEIN PHOSPHATASE 2C 59-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCCCACAGCCCGTCACTGGCCAGCAGC |
| Internal bar code: | CTCCCTCAGGGTACACAATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1224 |
| LEAP-Seq percent confirming: | 99.6235 |
| LEAP-Seq n confirming: | 1323 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGACGTGGTACGATGC |
| Suggested primer 2: | TTCGAATTCCCAATCCTCTG |