| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099196 |
| Chromosome: | chromosome 17 |
| Location: | 4040895 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729101 | PHC37 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTGACCTGTTCGGGAGTTCGTGGCAGC |
| Internal bar code: | TAGGGGTGGAAGTCGCAGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 98.9339 |
| LEAP-Seq n confirming: | 1856 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCTTCGAGTATGAAGC |
| Suggested primer 2: | CTTCCATGTACCGTACCGCT |