Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.099219 |
Chromosome: | chromosome_12 |
Location: | 216672 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g484900 | FAP83 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCATGCAATATAATTGTACAGCGCGCTTC |
Internal bar code: | CTGAGGGCTGCTCACTAGAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1113 |
LEAP-Seq percent confirming: | 99.5856 |
LEAP-Seq n confirming: | 5046 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTTGTCGGACTGAGGGAT |
Suggested primer 2: | CGTCTCAACGATGAACGAGA |