| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099219 |
| Chromosome: | chromosome 12 |
| Location: | 216672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484900 | FAP83 | (1 of 2) PF13879 - KIAA1430 homologue (KIAA1430); Flagellar Associated Protein 83 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCAATATAATTGTACAGCGCGCTTC |
| Internal bar code: | CTGAGGGCTGCTCACTAGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.5856 |
| LEAP-Seq n confirming: | 5046 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGTCGGACTGAGGGAT |
| Suggested primer 2: | CGTCTCAACGATGAACGAGA |