| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099258 |
| Chromosome: | chromosome 10 |
| Location: | 2736684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438400 | LTH2 | LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGTGAGGCGATGTAGCCGTGCGGAGC |
| Internal bar code: | TAGTGAGTAGTGGGCGAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.2545 |
| LEAP-Seq n confirming: | 2929 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTTGAGCGACTTGGTCT |
| Suggested primer 2: | ATCGTGAAAACACCTCTGGG |