Insertion junction: LMJ.RY0402.099265_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.099265
Chromosome:chromosome 9
Location:2704346
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g389650 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGCATGTACGGTGACTGGAGGTACGGT
Internal bar code:GGTTGATTCGGAATCGTGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:481
LEAP-Seq percent confirming:98.1752
LEAP-Seq n confirming:269
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTCCATCGTGCAATTT
Suggested primer 2:GACCAGTGATACTCGGGAGC