| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099267 |
| Chromosome: | chromosome 1 |
| Location: | 4335206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029400 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTCCTCCTGCAGCGACGCCGGCTCGCG |
| Internal bar code: | TTTAGCCGAGTCGAAGCTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.6436 |
| LEAP-Seq n confirming: | 4473 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGTCTATCCGCGTTAGC |
| Suggested primer 2: | AGTTTCGGGTTGAACCACAC |