| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099312 |
| Chromosome: | chromosome 6 |
| Location: | 3943634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278188 | NUOB18 | NADH:ubiquinone oxidoreductase 10 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCACGACCAATAGCTTGCGCCTGCGT |
| Internal bar code: | CAGAGGCCGAAGAGTACCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 41.6303 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 401 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTGTGTCCACCTCTCT |
| Suggested primer 2: | AATATGGCGGATAGGTTCCC |