| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099321 |
| Chromosome: | chromosome 5 |
| Location: | 1305345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244900 | (1 of 1) PF01594 - Domain of unknown function DUF20 (UPF0118) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGGCATACGGGCGCACATCCTCACGTG |
| Internal bar code: | GGGCGGGGAAAGTTCTAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1224 |
| LEAP-Seq percent confirming: | 98.5806 |
| LEAP-Seq n confirming: | 4306 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACGCGCACTGTCTGTAT |
| Suggested primer 2: | GGCAAATGACCTCAACCTGT |