| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099322 |
| Chromosome: | chromosome 7 |
| Location: | 1978425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325755 | (1 of 1) PTHR33606:SF3 - PROTEIN YCII | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCCTGGTGGTTGGTGCAGTCTGGATC |
| Internal bar code: | AGCTTCTGATGCGGTATCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.4743 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCTAGCACCCAGTGAG |
| Suggested primer 2: | CTTCGCGGTAGTTTCTCCAG |