| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099330 |
| Chromosome: | chromosome 3 |
| Location: | 4303496 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174700 | (1 of 1) 3.1.22.1 - Deoxyribonuclease II / Lysosomal DNase II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACACCCAGCGTGCATTGGCAGGCATTC |
| Internal bar code: | TGGAACGACCACTGGCCCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 63.4901 |
| LEAP-Seq n confirming: | 1026 |
| LEAP-Seq n nonconfirming: | 590 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGCCCAAGACATTTAG |
| Suggested primer 2: | CCTTCATCCCTGATTCCTCA |