| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099334 |
| Chromosome: | chromosome 3 |
| Location: | 8284245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199250 | CYG51 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACTCGGCCGTTGAAGTGTGCAGCGCGG |
| Internal bar code: | CATTCTAAAATGGGCTAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.8725 |
| LEAP-Seq n confirming: | 6267 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGTCCTCAAGCGTGTT |
| Suggested primer 2: | TTCACGCAGTTGCAACATTT |