| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099337 |
| Chromosome: | chromosome 2 |
| Location: | 8475118 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141786 | PDE25 | 3\',5\'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGGGAATGTACGGCTCGTGCACACTA |
| Internal bar code: | TCTCGAGTTACTGGTTGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 99.784 |
| LEAP-Seq n confirming: | 3234 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGCCTACATACCCGAAC |
| Suggested primer 2: | AAAGCAACCAGCAGTCCATC |