| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099353 |
| Chromosome: | chromosome 3 |
| Location: | 3852287 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170800 | (1 of 1) PTHR12231//PTHR12231:SF138 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTACACCGCAGTAATAAAGGGAGTCCAT |
| Internal bar code: | TACTGGGAGGGCGGGCTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 98.8809 |
| LEAP-Seq n confirming: | 2739 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCAAGGTGTTTGGCTTC |
| Suggested primer 2: | GAATACGGTACCGCACGACT |