| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099356 |
| Chromosome: | chromosome 5 |
| Location: | 2775502 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237000 | MAP1D | Methionine aminopeptidase; (1 of 1) PTHR10804:SF82 - METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTCGGTGATGACGGCCCGGCTCGACTC |
| Internal bar code: | CACGGTGCCATTTATTCAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 297 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGATGGTTGGCTCTGGTT |
| Suggested primer 2: | TCTCAGGAGCAGAACAGGGT |