| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099389 |
| Chromosome: | chromosome 1 |
| Location: | 3313745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020918 | PREP1 | (1 of 1) PTHR11851:SF68 - PRESEQUENCE PROTEASE 1, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Presequence protease 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCAGCAGTACCGCCGCACTGCTTGTAC |
| Internal bar code: | CAGGGAATAGTATGTGCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.8057 |
| LEAP-Seq n confirming: | 7191 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATTCACCTCCTGTCAGT |
| Suggested primer 2: | GATAAGGGTAGACAGGGGGC |