Insertion junction: LMJ.RY0402.099395_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.099395
Chromosome:chromosome 9
Location:7515118
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g414050 Pontin,RVB1,RUVBL1 Nucleoside-triphosphatase RuvB-like protein; (1 of 1) K04499 - RuvB-like protein 1 (pontin 52) (RUVBL1, RVB1, INO80H) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAATGTAACGAAAGATGACTTGAAGCCA
Internal bar code:CCTCGGTATAGGTACGTGGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:98.4375
LEAP-Seq n confirming:126
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGAGGTACTTGTCGCCGC
Suggested primer 2:CCTCGCCTTGTCTAATCTGC