| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099403 |
| Chromosome: | chromosome 9 |
| Location: | 7814347 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416500 | CGL151 | Conserved in the Green Lineage; (1 of 2) IPR026939 - At2g23090-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTTGCAAGGACTGCAGGTGGGCAGAA |
| Internal bar code: | CTGGGTCTTGGACACGGATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.6472 |
| LEAP-Seq n confirming: | 3389 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATGCGAGGGGTAGTCAC |
| Suggested primer 2: | GCCTAAGGTCGAGTTTGCAG |