Insertion junction: LMJ.RY0402.099410_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.099410
Chromosome:chromosome 15
Location:386051
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g636050 CPLD31 Conserved in the Plant Lineage and Diatoms; (1 of 2) PTHR35514//PTHR35514:SF1 - FAMILY NOT NAMED // THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGAGACGCATGGTTGAAAGCTGCGAA
Internal bar code:GTTGGGCTCGGGCAATGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:834
LEAP-Seq percent confirming:99.1154
LEAP-Seq n confirming:2465
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGGAGTAGTCAAGAGGC
Suggested primer 2:ACGTGTGTGATGCGATGAAT