| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099420 |
| Chromosome: | chromosome 1 |
| Location: | 1713000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009200 | POB29 | Proteome of basal body 29; (1 of 2) IPR002083//IPR008974 - MATH/TRAF domain // TRAF-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACCCGGCCGGCCTGAGCGCGCAGGGG |
| Internal bar code: | CTGTTCGCTGTGTACGCTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 99.7528 |
| LEAP-Seq n confirming: | 807 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATGCGTCAGTGCTCATT |
| Suggested primer 2: | TGTCGCCTCTTATCATGCTG |