| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099431 |
| Chromosome: | chromosome 14 |
| Location: | 3397501 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630400 | L-galactose dehydrogenase; (1 of 1) 1.1.1.316 - L-galactose 1-dehydrogenase / L-galDH | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAGGGGGCATCCGGGGGCACGTGTGAG |
| Internal bar code: | GAGCATCCTGCAGGTTGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 99.1723 |
| LEAP-Seq n confirming: | 1318 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACACTACACCCGTTTCC |
| Suggested primer 2: | TTGAACTCCTCACCCACCTC |