| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099440 |
| Chromosome: | chromosome 9 |
| Location: | 1999749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394550 | RPPK2 | ribose-phosphate pyrophosphokinase; (1 of 1) PTHR10210//PTHR10210:SF45 - RIBOSE-PHOSPHATE PYROPHOSPHOKINASE // RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 3, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCAAGGGCGGAGGGGGAGGCAGCGTCA |
| Internal bar code: | CCGGGTGTGGAATCGGCTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.6627 |
| LEAP-Seq n confirming: | 591 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTAGCCTCAGCCAAAC |
| Suggested primer 2: | GTCTTGCCCTAGCCTCTCCT |