| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099476 |
| Chromosome: | chromosome_8 |
| Location: | 1951163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367550 | FAP291 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TATCGCCGAAAGGCTGGGTGCTCTCTGGTA |
| Internal bar code: | AATGGCGCCCATGGGCTATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.1178 |
| LEAP-Seq n confirming: | 1573 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCTCACGTCTTCCTCG |
| Suggested primer 2: | TGCACAGATAGCAGTCAGGG |