Insertion junction: LMJ.RY0402.099478_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.099478
Chromosome:chromosome 13
Location:1983583
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g576600 Conserved Protein with Similarity to Catalytic domain of Protein Tyrosine Kinases; (1 of 1) PTHR23257//PTHR23257:SF405 - SERINE-THREONINE PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-LIKE-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGTGGCGGTCGTGATCTGCGCATGACA
Internal bar code:AAAGTGTTCCGTCGCCGGTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1229
LEAP-Seq percent confirming:99.6136
LEAP-Seq n confirming:2836
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTAGTTCCCACTGAGTC
Suggested primer 2:ACAACCGCAACTCCTACTCG