| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099486 |
| Chromosome: | chromosome 7 |
| Location: | 6333545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357200 | UGD1,EZY12 | Similar to UDP-Glucose Dehydrogenase; (1 of 3) 1.1.1.22 - UDP-glucose 6-dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGCGCTTTGTGGAGCGCGTCATCGG |
| Internal bar code: | GTGGTCCCCTGGGGATGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 97.9687 |
| LEAP-Seq n confirming: | 1688 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGTTCGTGTCCGTCAA |
| Suggested primer 2: | TCGCCTAACCTCACCGTTAC |