| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099488 |
| Chromosome: | chromosome 7 |
| Location: | 226442 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313700 | TSX2,GTS2,TSE2,GTS2:GLTX | Glutamyl/glutaminyl-tRNA synthetase; (1 of 2) 6.1.1.17 - Glutamate--tRNA ligase / Glutamyl-tRNA synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGAGACACTGGCGGCGCCCGAGGCCA |
| Internal bar code: | AAATTTGCCATAGCATCATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 78.7056 |
| LEAP-Seq n confirming: | 377 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTGCCCTACTCTCTCA |
| Suggested primer 2: | TGATCAACTTCCTGTCGCTG |