| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099504 |
| Chromosome: | chromosome 16 |
| Location: | 211301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694800 | GT90F31,GT90-31 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 31 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACTCACTCACACTCACTCACGTACCTT |
| Internal bar code: | CCCGGACCTGGTCGGGTAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 99.176 |
| LEAP-Seq n confirming: | 1685 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGCAGAGCACAAGGAA |
| Suggested primer 2: | GGTTTTGGCAACATCGACTT |