| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099508 |
| Chromosome: | chromosome 5 |
| Location: | 632844 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232004 | (1 of 1) IPR012677//IPR014720 - Nucleotide-binding alpha-beta plait domain // Double-stranded RNA-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGAAGTGAAGTCGTAGGCCGGTACAGT |
| Internal bar code: | AGAGTCTCGCCTAACCTCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GGGTGTTGGCTGGATGTACT |