| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099516 |
| Chromosome: | chromosome 11 |
| Location: | 1294554 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGACCTGCCCTCTCCCTCGCCTGAGGC |
| Internal bar code: | TGGCCTTATCGGAGCCGCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.6059 |
| LEAP-Seq n confirming: | 1011 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAACGGTGCCATAATCA |
| Suggested primer 2: | TCCTCATCATCATCGCCATA |