| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099533 |
| Chromosome: | chromosome 14 |
| Location: | 3380510 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630250 | CYCR1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTAACAACCAAACAGCTAGGTCGCATC |
| Internal bar code: | GGGCAATATCGGATACAGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 96.0159 |
| LEAP-Seq n confirming: | 241 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCTCTATCCCTTGCTG |
| Suggested primer 2: | GTGTGAGCGTGTAAGCGTGT |