| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099555 |
| Chromosome: | chromosome 16 |
| Location: | 4373514 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671450 | FAO12 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF150 - FAD NAD BINDING OXIDOREDUCTASES // OXIDOREDUCTASE TDA3-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCCGCGCCTGTACCTGCTGGCTGCGC |
| Internal bar code: | ATCTGGCTCCATGGTGAGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 54.8273 |
| LEAP-Seq n confirming: | 619 |
| LEAP-Seq n nonconfirming: | 510 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAATCGAGAGGTGTGCTG |
| Suggested primer 2: | ATTTGAATCCGGTGAACAGC |