| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099581 |
| Chromosome: | chromosome 10 |
| Location: | 5493626 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459050 | (1 of 4) 3.4.21.83 - Oligopeptidase B / Protease II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATATTGTTGGGATGATGATGTAGAGGTCG |
| Internal bar code: | GTAGCCGGACCTATGTTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.1209 |
| LEAP-Seq n confirming: | 451 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTGTTACCCCTGCGGA |
| Suggested primer 2: | GGTGTCGTACAGTTGCATGG |