| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099612 |
| Chromosome: | chromosome 1 |
| Location: | 5189427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036100 | (1 of 1) K11790 - denticleless (DTL, CDT2, DCAF2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGCCCTTCTCCCAAGCTTGGCACTGC |
| Internal bar code: | TGAAGGGGTGCATTTAAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 94.9336 |
| LEAP-Seq n confirming: | 4216 |
| LEAP-Seq n nonconfirming: | 225 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTCAAGATTTCATGCCG |
| Suggested primer 2: | GTTGATGTGATTGAGCACCG |