Insertion junction: LMJ.RY0402.099619_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.099619
Chromosome:chromosome 12
Location:2899600
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g501950 PPP39 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTTTCGTTTGGGCTGGCATCTATACAT
Internal bar code:AACTCCTCTTCAGTTTGAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:91.0302
LEAP-Seq n confirming:4648
LEAP-Seq n nonconfirming:458
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGAAGCGTCCAAAACTT
Suggested primer 2:CGTGGAAGAGAATCGGACAT