| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099641 |
| Chromosome: | chromosome 7 |
| Location: | 1271687 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321700 | ADH3 | Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF281 - ALCOHOL DEHYDROGENASE RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGGTCGGCGGGGCCTCGCTCCACCC |
| Internal bar code: | TGATGGGCAATGGGGCGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 98.0023 |
| LEAP-Seq n confirming: | 1717 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGATGACGGTGTTGAGT |
| Suggested primer 2: | CTAGCTATTCCCCACACCCA |