| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099713 |
| Chromosome: | chromosome_8 |
| Location: | 2549208 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g372350 | FAP188 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGTGTGTTGATGCGTGTGTGTATATATA |
| Internal bar code: | CTATTCGCATATTCCGTTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.7612 |
| LEAP-Seq n confirming: | 5849 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCTCGTACACTGCTCT |
| Suggested primer 2: | GGTCTAGCCTTGATTCGCTG |