| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099716 |
| Chromosome: | chromosome 12 |
| Location: | 6899830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560700 | MDJ1B,CDJ6 | (1 of 2) PTHR24078//PTHR24078:SF290 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; Mitochondrial DnaJ-like protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGCGGGTGCGGCGACCGGCGCGACG |
| Internal bar code: | TGTATCCTAAGTATGTAAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.8968 |
| LEAP-Seq n confirming: | 3873 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGCCCGCATATACCTG |
| Suggested primer 2: | CCTTTCTGCCTACAGCTTGG |