| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099726 |
| Chromosome: | chromosome 3 |
| Location: | 5730621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188100 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGAGAGGCATGGGTGGGATTGAAGGAG |
| Internal bar code: | AGTCTCTCTATTTCGGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 97.4778 |
| LEAP-Seq n confirming: | 1430 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAGTCAGCTTCTCCACC |
| Suggested primer 2: | CTTCTGACGTTGCATCTGGA |