| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099726 |
| Chromosome: | chromosome 9 |
| Location: | 944349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401000 | SNE3 | UDP-N-acetylglucosamine/UDP-glucose 4-epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGAGGGGTGCTTTCATCACTGCCGAC |
| Internal bar code: | GATGAGCCCGGCGACGGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 98.8231 |
| LEAP-Seq n confirming: | 2771 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCATAAGTACCGCTTAG |
| Suggested primer 2: | CTTCATGTCATCGTCATCGG |