| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099732 |
| Chromosome: | chromosome 16 |
| Location: | 4975579 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685250 | SUB11 | (1 of 5) PTHR10795//PTHR10795:SF157 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGCATTCGACGGCGCCGCATAGACGG |
| Internal bar code: | GCATGGCGTATAGGCGTCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACCTGCCTTGGTTGAT |
| Suggested primer 2: | AACGTGACACTGGTGCTCTG |