| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099739 |
| Chromosome: | chromosome 12 |
| Location: | 4478674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521650 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTGCTGGTGTACCTGCCGGGCATCG |
| Internal bar code: | TCTGGACTGGAGGGTCGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 86.9721 |
| LEAP-Seq n confirming: | 53046 |
| LEAP-Seq n nonconfirming: | 7946 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCGTCACTACCGCCAC |
| Suggested primer 2: | CACGCATTACCATTGTCCAG |