| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099746 |
| Chromosome: | chromosome 13 |
| Location: | 4043406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591200 | HTB38,HTB22 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATAAGGAATGTGTTGGAGTAGGGTTGT |
| Internal bar code: | AAGGACTCGATCGGCGTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 99.524 |
| LEAP-Seq n confirming: | 6273 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACAATGAAACAATGCGG |
| Suggested primer 2: | ACGCTCCTGTGAGTCTGGAT |