Insertion junction: LMJ.RY0402.099747_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.099747
Chromosome:chromosome 17
Location:4646680
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g733250 FKB16D,FKB16-4,FKB7 (1 of 1) PTHR10516:SF323 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP16-4, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTAATCACATGGTGTTGTATTGCAAGAG
Internal bar code:AAGGTCGCTTGGCCGGGTTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:889
LEAP-Seq percent confirming:99.641
LEAP-Seq n confirming:5274
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCAGTTCACCCCACCAG
Suggested primer 2:AAGGACAGCGAGCGTACAGT