| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099757 |
| Chromosome: | chromosome 12 |
| Location: | 5316062 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529150 | PPP40,PP2C1,FAP314 | Flagellar Associated Protein 314; (1 of 1) PTHR13832:SF352 - PROTEIN PHOSPHATASE 2C 59-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCAATCCCGGCTTCCCCTGCGCTCTCT |
| Internal bar code: | GCTCGTCGGGGTTCTGTTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 85.5931 |
| LEAP-Seq n confirming: | 4741 |
| LEAP-Seq n nonconfirming: | 798 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTTCAGCACCACTACA |
| Suggested primer 2: | GTGTTGACGTGGTACGATGC |