Insertion junction: LMJ.RY0402.099776_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.099776
Chromosome:chromosome_10
Location:2294117
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g434600 FAP148 Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGAAGCCTTGACACGCCTTGACGCCCACCG
Internal bar code:CGTGGGGGGACTTAGGATGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:538
LEAP-Seq percent confirming:98.9776
LEAP-Seq n confirming:2517
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGCAGAATTGAGACGA
Suggested primer 2:CACTCATTGTGGTTATGGCG