| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099799 |
| Chromosome: | chromosome 5 |
| Location: | 3187932 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATCGGCCCAACACCATCATTCTTAAGC |
| Internal bar code: | CTTCGGATGGAGACAGGTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.3872 |
| LEAP-Seq n confirming: | 5190 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTTGTGGGCAGATGGTT |
| Suggested primer 2: | TGACAAAACTGCTGACTGCC |