| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099841 |
| Chromosome: | chromosome 12 |
| Location: | 3532671 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513300 | CGL23 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTACAGACGGGCTGGGCGGCGGCAGTAG |
| Internal bar code: | TAGCGCGACCCTACGTGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 97.0641 |
| LEAP-Seq n confirming: | 1091 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGTCAGGCGGTGATAGG |
| Suggested primer 2: | AGCCCACATAATCGTTCTGG |