| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099848 |
| Chromosome: | chromosome 3 |
| Location: | 7675724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203850 | ATS1 | ATP-sulfurylase; (1 of 2) 2.7.7.4 - Sulfate adenylyltransferase / Sulfurylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCTGCCGGACCCCCGCTGCGCCGTGCA |
| Internal bar code: | TGGTTCCGCTAAGATCCGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 99.2692 |
| LEAP-Seq n confirming: | 9780 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTTCCTTCCCAATC |
| Suggested primer 2: | CTTCATGAACAAGGCGGAGT |