| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099854 |
| Chromosome: | chromosome 7 |
| Location: | 161611 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313250 | (1 of 1) 3.6.3.17//3.6.3.25 - Monosaccharide-transporting ATPase // Sulfate-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGAAAAGTAGGCGGCTGCCGTGCGGCC |
| Internal bar code: | ACCTGCTCGGTTCTATGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.3289 |
| LEAP-Seq n confirming: | 1036 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCATGTGACCAGTTGC |
| Suggested primer 2: | CAAGTGGACCTACATGGGCT |