| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099890 |
| Chromosome: | chromosome 5 |
| Location: | 1628892 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243351 | (1 of 3) IPR000477//IPR002156//IPR012337 - Reverse transcriptase domain // Ribonuclease H domain // Ribonuclease H-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATGTAGCGACGTGCCGACGGGTCCAG |
| Internal bar code: | TTCAACGCCTTTGTCCAGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.0423 |
| LEAP-Seq n confirming: | 2482 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTAGAGCCCCTGTTGAG |
| Suggested primer 2: | CCCTAGATCCTTCCTCAGGG |